宋金召
- 电子邮件：songjinzhao@him.cas.cn
个人简历
宋金召，中国科学院医学所特聘研究员、国科大杭州高等研究院双聘教授，博士毕业于中国科学院化学研究所。自2013年起，先后在加州大学洛杉矶分校医学院和宾夕法尼亚大学工程学院从事博士后研究。回国前，担任宾夕法尼亚大学研究助理教授，作为PI和co-PI成功获得了NIH K01和NIH R21两项科研项目的资助和一项创业孵化基金的资助，总金额～130万美元，并获NIH“科学家发展奖”、美国临床化学会“精准医疗青年科学家奖”。回国后，入选了浙江省海外高层次人才引才计划创新长期项目和浙江省卫生高层次创新人才项目。
主要致力于智能分子诊断方法、试剂、检测仪器及肿瘤标志物研究，通过分析化学、分子生物学以及工程技术（机械、电子、计算机、人工智能等）的多学科交叉研究发展了一系列分子诊断的新原理、新方法和新装置。1）首次将原核Argonaute蛋白（pAgo）作为一种可编程核酸内切酶引入到分子诊断领域中，建立了高灵敏的稀有突变基因富集及检测方法，成功用于了胰腺癌的液体活检，并丰富了分子诊断的“工具酶箱”。2）建立了多个即时分子诊断体系，针对寨卡和新冠病毒所建立的即时核酸快检方法已被用于此两种病毒的检测。近年来，以第一/通讯作者在分子诊断领域top期刊发表论文16篇，包括Nucleic Acids Res.、Angew. Chem. Int. Ed.、Clin. Chem.、Biosensors & Bioelectronics、Anal. Chem.等，申请国际、国内专利15项，已推出了分子POCT核酸快检产品和超灵敏肿瘤液体活检产品，并已成立公司（杭州逸检科技）进行转化推广。
研究方向
IoT智能分子诊断；高灵敏液体活检方法及标志物研究；居家智能检测系统研究
承担科研项目情况
1. Smartphone-based mobile detection platform for lung cancer detection in China，2019-09-16~2024-06-30，450万，美国国立卫生研究院，主持；
2.Smartphone-based detection of cancer mutant alleles in biological samples，2019-08-01~2021-04-30，260万，美国国立卫生研究院，主持；
3.医学所PI启动经费，2020-10-01~2025-09-30，300万，中科院杭州医学所，主持；
4. 浙江省卫生高层次创新人才项目，2020-10-01~2020-09-30，40万，浙江省，主持；
5. 浙江省创新创业团队，2022-10-01~2026-09-30，40万，浙江省，参与；
6. 乳腺癌筛查早诊多组学标志物与一体化检测技术研发，2025-01-01~2026-12-31，40万，浙江省，参与；
7. 钱塘交叉基金，2021-09-01~2022-08-30，5万，中科院杭州医学所，主持。
代表论著
1. Fengyi Dai, Tao Zhang, Feibiao Pang, Tianjiao Jiao, Kaizheng Wang, Zhanfang Zhang, Nuo Wang, Zhiwei Xie, Yanchong Zhang, Zihao Wang, Zhiguang Chen, Mingxia Yu*, Hongping Wei*, Jinzhao Song*. A compact, palm-sized isothermal fluorescent diagnostic intelligent IoT device for personal health monitoring and beyond via one-tube/one-step LAMP-CRISPR assay, Biosensors and Bioelectronics 270, 116945 (2025).
2. Kaizheng Wang, Tao Zhang, Tianjiao Jiao, Feibiao Pang, Fengyi Dai, Zhanfang Zhang, Xiang Li, Yao Chen, Hongyu Zhang, Zhiguang Chen, Jinzhao Song*. Mini-Program Enabled IoT Intelligent Molecular Diagnostic Device for Co-Detection and Spatiotemporal Mapping of Infectious Disease Pathogens, Analytical Chemistry 96 (33), 13494-13503 (2024).
3. Feibiao Pang, Tao Zhang, Fengyi Dai, Kaizheng Wang, Tianjiao Jiao, Peng Hu*, Jinzhao Song*. A Handheld Isothermal Fluorescence Detection Device for Duplex Visualization of Aquatic Pathogens via Enhanced One-Pot LAMP-PfAgo Assay. Biosensors and Bioelectronics 254, 116187 (2024).
4. Yue Shen, Xiaoling Zhang, Liyi Zhang, Zuoying Zhang, Bao Lyu, Qian Lai, Qinglin Li, Yuhua Zhang, Jieer Ying*, Jinzhao Song*. Performance evaluation of a CRISPR Cas9-based selective exponential amplification assay for the detection of KRAS mutations in plasma of patients with advanced pancreatic cancer. Journal of Clinical Pathology, 2023/9/7 (2023).
5. Junman Chen#, Tian Qiu#, Michael G. Mauk, Zheng Su, Yaguang Fan, Dennis J. Yuan, Qinghua Zhou, Youlin Qiao, Haim H. Bau, Jianming Ying*, Jinzhao Song*. Programmable endonuclease combined with isothermal polymerase amplification to selectively enrich for rare mutant allele fractions. Chinese Chemical Letters, DOI: 10.1016/j.cclet.2021.11.065 (2021).
6. Junman Chen, Tian Qiu, Michael G. Mauk, Yaguang Fan, Yong Jiang, Jianming Ying, Qinghua Zhou, Youlin Qiao, Haim H. Bau, Jinzhao Song*. CRISPR Cas9-Mediated Selective Isothermal Amplification for Sensitive Detection of Rare Mutant Alleles. Clinical Chemistry, 67, 1569-1571 (2021).
7. Jinzhao Song*, Mohamed El-Tholoth, Yize Li, Jevon Graham-Wooten, Yining Liang, Juan Li, Weijian Li, Susan R. Weiss, Ronald G. Collman, and Haim H. Bau. Single and Two-Stage, Closed-Tube, Point of Care, Molecular Detection of SARS-CoV-2. Anal. Chem., 93, 13063–13071 (2021).
8. Jinzhao Song*, J.W.H., Michael G. Mauk, Neha Bhagwat, Jacob E. Till, Lotte T. Azink, Jing Peng, Moen Sen, Junman Chen, Jazmine Mays, Erica Carpenter, John van der Oost*, and Haim H. Bau*. Highly specific enrichment of rare nucleic acid fractions using Thermus thermophilus argonaute with applications in cancer diagnostics. Nucleic Acids Research, 48(4): e19 (2020).
9. Jinzhao Song*, Michael Joseph Powell, Wei Liu, Junman Chen, Haim Bau. Highly sensitive detection of rare mutant alleles by combining argonaute-based enrichment and XNA-PCR. Journal of Clinical Oncology 37 (15_suppl), e14605-e14605 (2019).
10. Jinzhao Song, Vikram Pandian, Michael G. Mauk, Haim H. Bau, Sara Cherry, Laurence C. Tisic and Changchun Liu. Smartphone-based Mobile Detection Platform for Rapid Molecular Diagnostics and Spatiotemporal Disease Mapping. Anal. Chem., 90, 4823–4831 (2018).
11. Jinzhao Song, Changchun Liu, Michael G. Mauk, Jing Peng, Thomas Schoenfeld, and Haim H. Bau*. A Multifunctional Reactor with Dry-Stored Reagents for Enzymatic Amplification of Nucleic Acids. Anal. Chem., 90, 1209–1216 (2018).
12. Jinzhao Song, Changchun Liu, Michael G. Mauk, Shelley C. Rankin, Robert M. Greenberg, and Haim H. Bau*. Two-stage isothermal enzymatic amplification for concurrent multiplex molecular detection. Clinical Chemistry, 63, 714-722 (2017).
13. Jinzhao Song, Michael Mauk, Brent Hackett, Sara Cherry, Haim H. Bau and Changchun Liu*. Instrument-Free Point-of-Care Molecular Detection of Zika Virus. Anal. Chem., 88 (14), 7289-7294 (2016).
14. Jinzhao Song, Changchun Liu, Swarna Bais, Michael G. Mauk, Haim H. Bau, Robert M. Greenberg*. Molecular Detection of Schistosome Infections with a Disposable Microfluidic Cassette. PLos Neglect Trop D. 9(12), doi: ARTN e0004318 (2015).
15. Jinzhao Song, Changchun Liu, Thomas J Nolan, Michael Mauk, James B Lok, Haim H Bau, Robert M Greenberg. Molecular detection of Schistosome and other parasitic helminth infections on a disposable microfluidic cassette. American Journal of Tropical Medicine and Hygiene 93 (4), 544-544. (2015)
16. Jinzhao Song, Jiangyan Zhang, Fengting Lv*, Yongqiang Cheng, Bing Wang, Liheng Feng, Libing Liu, Shu Wang*. Multiplex detection of DNA mutations by fluorescence fingerprint spectrum technique. Angew. Chem. Int. Ed. 52, 13020-13023 (2013).
17. Baoling Xing#, Jinzhao Song#, Sumei Ge, Zhenghua Tang, Menglu Liu, Qiong Yang*, Fengting Lv, Libing Liu, Shu Wang*. Multiplex detection of KRAS and BRAF mutations using cationic conjugated polymers. Sci. Bull. 58, 873-878 (2013).
18. Jinzhao Song, Fengting Lv*, Gaomai Yang, Libing Liu, Qiong Yang, Shu Wang*. Aptamer-based polymerase chain reaction for ultrasensitive cell detection. Chem. Commun. 48, 7465-7467 (2012).
19. Jinzhao Song, Qiong Yang*, Fengting Lv, Libing Liu, Shu Wang*. Visual detection of DNA mutation using multicolor fluorescent coding. ACS Appl. Mater. Interfaces 4, 2885-2890 (2012).
20. Jinzhao Song, Zhengping Li*, Yongqiang Cheng, Chenghui Liu. Self-aggregation of oligonucleotide-functionalized gold nanoparticles and its applications for highly sensitive detection of DNA. Chem. Commun. 46, 5548-5550 (2010).
获奖及荣誉
获奖：1. 互联网+全国总决赛金奖优秀指导教师；2.美国临床化学会“精准医疗青年科学家奖” ；3.NIH科学家发展奖 专利：• Compositions, kits, and methods to detect HIV virus. Jinzhao Song, Haim H. Bau, Changchun Liu, Frederic D. Bushman, International patent, WO 2017099801 A1
• Multiple Stage Isothermal Enzymatic Amplification. Jinzhao Song, Haim H. Bau, Changchun Liu, Michael G. Mauk, International patent, WO2017123921 A1
• Compositions And Methods For Zika Virus Detection. Jinzhao Song, Haim H. Bau, Changchun Liu, Michael G. Mauk, United States Provisional Patent Application No. 62/346,375
• Enrichment of Nucleic Acids. Jinzhao Song, Haim H. Bau, John van der Oost, Jorrit Hegge, Changchun Liu, Michael G. Mauk, International patent, WO2019178346 A1
• The method of visual and multiplex detection of DNA mutations based on cationic conjugated polymer. Shu Wang, Qiong Yang, Jinzhao Song, China patent, 201110369994.0
• Programmable Enzyme-Assisted Selective Exponential Amplification For Sensitive Detection Of Rare Mutant Alleles. Jinzhao Song, Haim H. Bau, Junman Chen, U.S. Provisional Patent Application No. 63/225,370.
